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Biology Practice Tests

These Grade 9 Biology practice tests introduce the study of life through questions about life processes, cells, organelles, biomolecules, enzymes, photosynthesis, cellular respiration, homeostasis, adaptation, and scientific investigation.

Skills covered

  • Recognizing characteristics of life, levels of organization, homeostasis, metabolism, reproduction, and adaptation
  • Identifying cell structures and explaining the functions of the nucleus, membrane, mitochondria, chloroplasts, ribosomes, and other organelles
  • Understanding carbohydrates, proteins, lipids, nucleic acids, enzymes, water, pH, and biological reactions
  • Connecting photosynthesis, cellular respiration, ATP, fermentation, matter, and energy in living systems
  • Analyzing hypotheses, variables, controls, repeated trials, models, graphs, conclusions, and scientific communication

How to use these practice tests

Identify the level of organization or process, connect structures to functions, track matter and energy, and use evidence from experiments or graphs. Review each explanation to strengthen biology vocabulary and scientific reasoning.

For more Grade 9 Science practice, return to the Grade 9 Science category.

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Grade 9 Biology Practice Tests

Practice Grade 9 Science questions about characteristics of life, cells, organelles, biomolecules, enzymes, photosynthesis, cellular respiration, homeostasis, and scientific investigation.

Choose the best answer. Identify the biological system or process, apply the relevant principle, and use evidence when interpreting an experiment or model.

10 Questions
0/10 Questions0 %
Correct 0
Incorrect 0
Unanswered 10
1. QuestionMultiple choice

What is the main purpose of photosynthesis?

Please select an answer first.

Correct answer: A

The correct answer is To convert light energy into chemical energy stored in glucose..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Photosynthesis stores light energy in glucose using carbon dioxide and water.
  3. Check the answer: The correct answer is To convert light energy into chemical energy stored in glucose..

Photosynthesis stores light energy in glucose using carbon dioxide and water.

2. QuestionMultiple choice

Which substance is a reactant in photosynthesis?

Please select an answer first.

Correct answer: B

The correct answer is Carbon dioxide..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Plants use carbon dioxide and water, with light energy, to produce glucose and oxygen.
  3. Check the answer: The correct answer is Carbon dioxide..

Plants use carbon dioxide and water, with light energy, to produce glucose and oxygen.

3. QuestionMultiple choice

Where does photosynthesis occur in plant cells?

Please select an answer first.

Correct answer: C

The correct answer is Chloroplasts..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Chloroplasts contain chlorophyll and the internal membranes where photosynthesis occurs.
  3. Check the answer: The correct answer is Chloroplasts..

Chloroplasts contain chlorophyll and the internal membranes where photosynthesis occurs.

4. QuestionMultiple choice

What is the main purpose of cellular respiration?

Please select an answer first.

Correct answer: D

The correct answer is To release usable energy from food molecules..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Cellular respiration breaks down food molecules and transfers energy to ATP.
  3. Check the answer: The correct answer is To release usable energy from food molecules..

Cellular respiration breaks down food molecules and transfers energy to ATP.

5. QuestionMultiple choice

Which molecule is the main usable energy carrier in cells?

Please select an answer first.

Correct answer: A

The correct answer is ATP..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: ATP transfers usable energy to power cellular work.
  3. Check the answer: The correct answer is ATP..

ATP transfers usable energy to power cellular work.

6. QuestionMultiple choice

Which gas is produced as a product of photosynthesis?

Please select an answer first.

Correct answer: B

The correct answer is Oxygen..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Oxygen is released when water molecules are split during the light-dependent reactions.
  3. Check the answer: The correct answer is Oxygen..

Oxygen is released when water molecules are split during the light-dependent reactions.

7. QuestionMultiple choice

Which gas is produced as a waste product of aerobic cellular respiration?

Please select an answer first.

Correct answer: C

The correct answer is Carbon dioxide..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Cells release carbon dioxide when carbon compounds are broken down during respiration.
  3. Check the answer: The correct answer is Carbon dioxide..

Cells release carbon dioxide when carbon compounds are broken down during respiration.

8. QuestionMultiple choice

What is the relationship between photosynthesis and cellular respiration?

Please select an answer first.

Correct answer: D

The correct answer is The products of one process can serve as reactants for the other..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Photosynthesis produces glucose and oxygen, while respiration uses glucose and oxygen and returns carbon dioxide and water.
  3. Check the answer: The correct answer is The products of one process can serve as reactants for the other..

Photosynthesis produces glucose and oxygen, while respiration uses glucose and oxygen and returns carbon dioxide and water.

9. QuestionMultiple choice

Why do cells need ATP?

Please select an answer first.

Correct answer: A

The correct answer is ATP provides energy for transport, movement, synthesis, and other cellular work..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: ATP supplies energy for many forms of cellular work.
  3. Check the answer: The correct answer is ATP provides energy for transport, movement, synthesis, and other cellular work..

ATP supplies energy for many forms of cellular work.

10. QuestionMultiple choice

What is fermentation useful for when oxygen is limited?

Please select an answer first.

Correct answer: B

The correct answer is It allows some cells to continue producing a small amount of ATP without oxygen..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Fermentation regenerates molecules needed for glycolysis, allowing limited ATP production without oxygen.
  3. Check the answer: The correct answer is It allows some cells to continue producing a small amount of ATP without oxygen..

Fermentation regenerates molecules needed for glycolysis, allowing limited ATP production without oxygen.

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