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Biology Practice Tests

These Grade 9 Biology practice tests introduce the study of life through questions about life processes, cells, organelles, biomolecules, enzymes, photosynthesis, cellular respiration, homeostasis, adaptation, and scientific investigation.

Skills covered

  • Recognizing characteristics of life, levels of organization, homeostasis, metabolism, reproduction, and adaptation
  • Identifying cell structures and explaining the functions of the nucleus, membrane, mitochondria, chloroplasts, ribosomes, and other organelles
  • Understanding carbohydrates, proteins, lipids, nucleic acids, enzymes, water, pH, and biological reactions
  • Connecting photosynthesis, cellular respiration, ATP, fermentation, matter, and energy in living systems
  • Analyzing hypotheses, variables, controls, repeated trials, models, graphs, conclusions, and scientific communication

How to use these practice tests

Identify the level of organization or process, connect structures to functions, track matter and energy, and use evidence from experiments or graphs. Review each explanation to strengthen biology vocabulary and scientific reasoning.

For more Grade 9 Science practice, return to the Grade 9 Science category.

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Grade 9 Biology Practice Tests

Practice Grade 9 Science questions about characteristics of life, cells, organelles, biomolecules, enzymes, photosynthesis, cellular respiration, homeostasis, and scientific investigation.

Choose the best answer. Identify the biological system or process, apply the relevant principle, and use evidence when interpreting an experiment or model.

10 Questions
0/10 Questions0 %
Correct 0
Incorrect 0
Unanswered 10
1. QuestionMultiple choice

Which type of biomolecule is made from amino acids?

Please select an answer first.

Correct answer: A

The correct answer is Protein..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Proteins are polymers built from chains of amino acids.
  3. Check the answer: The correct answer is Protein..

Proteins are polymers built from chains of amino acids.

2. QuestionMultiple choice

Which biomolecule is commonly used as a quick source of cellular energy?

Please select an answer first.

Correct answer: B

The correct answer is Carbohydrate..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Carbohydrates such as glucose can be broken down to provide usable energy.
  3. Check the answer: The correct answer is Carbohydrate..

Carbohydrates such as glucose can be broken down to provide usable energy.

3. QuestionMultiple choice

What is a major function of lipids?

Please select an answer first.

Correct answer: C

The correct answer is Long-term energy storage and formation of cell membranes..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Lipids store energy and are important components of phospholipid cell membranes.
  3. Check the answer: The correct answer is Long-term energy storage and formation of cell membranes..

Lipids store energy and are important components of phospholipid cell membranes.

4. QuestionMultiple choice

What do nucleic acids store and transmit?

Please select an answer first.

Correct answer: D

The correct answer is Genetic information..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: DNA and RNA are nucleic acids involved in storing and transmitting genetic instructions.
  3. Check the answer: The correct answer is Genetic information..

DNA and RNA are nucleic acids involved in storing and transmitting genetic instructions.

5. QuestionMultiple choice

What is an enzyme?

Please select an answer first.

Correct answer: A

The correct answer is A biological catalyst that speeds up a chemical reaction..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Enzymes lower activation energy and help reactions occur more quickly without being consumed.
  3. Check the answer: The correct answer is A biological catalyst that speeds up a chemical reaction..

Enzymes lower activation energy and help reactions occur more quickly without being consumed.

6. QuestionMultiple choice

What can happen to an enzyme when temperature becomes too high?

Please select an answer first.

Correct answer: B

The correct answer is Its shape can change, reducing its ability to function..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: High temperatures can denature an enzyme and alter the shape of its active site.
  3. Check the answer: The correct answer is Its shape can change, reducing its ability to function..

High temperatures can denature an enzyme and alter the shape of its active site.

7. QuestionMultiple choice

What is the substrate in an enzyme reaction?

Please select an answer first.

Correct answer: C

The correct answer is The reactant that binds to the enzyme..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: A substrate fits into an enzyme's active site and is changed during the reaction.
  3. Check the answer: The correct answer is The reactant that binds to the enzyme..

A substrate fits into an enzyme's active site and is changed during the reaction.

8. QuestionMultiple choice

Why is water important to living organisms?

Please select an answer first.

Correct answer: D

The correct answer is It acts as a solvent and participates in many cellular processes..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Water dissolves substances and supports transport and chemical reactions in cells.
  3. Check the answer: The correct answer is It acts as a solvent and participates in many cellular processes..

Water dissolves substances and supports transport and chemical reactions in cells.

9. QuestionMultiple choice

Which pH condition is most acidic?

Please select an answer first.

Correct answer: A

The correct answer is pH 2..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Lower pH values are more acidic; pH 2 is more acidic than neutral or basic values.
  3. Check the answer: The correct answer is pH 2..

Lower pH values are more acidic; pH 2 is more acidic than neutral or basic values.

10. QuestionMultiple choice

Why can a change in pH affect an enzyme?

Please select an answer first.

Correct answer: B

The correct answer is The change can alter the enzyme's shape and active site..

  1. Identify the biology concept: Determine whether the question asks about life processes, cells, matter, energy, regulation, or scientific reasoning.
  2. Apply the biological principle: Enzyme structure depends on chemical conditions, including pH, so extreme changes can reduce activity.
  3. Check the answer: The correct answer is The change can alter the enzyme's shape and active site..

Enzyme structure depends on chemical conditions, including pH, so extreme changes can reduce activity.

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